Cordyceps (Cordyceps militaris) is a medicinal fungus grown axenically on a sterile rice substrate rather than on its traditional insect host, prized for its content of cordycepin and other bioactive compounds.1

Inputs

  • Substrate: rice (fresh or aged), 40 g dry material per 500 mL culture bottle at 60% water content, sterilized at 121°C for 60 minutes.1
  • Spawn: 8-10% (w/w) grain spawn inoculated onto the cooled substrate surface.1
  • Spawn run: dark, 18-22°C, until white mycelium fully covers the substrate.1
  • Fruiting initiation: 25±1°C, diffuse light at 800-1,000 lux on a 12-hour light/dark cycle, 70-80% relative humidity.1
  • Fruiting: once primordia form, holes are punched in the bottle covering and humidity raised to 85-95% at 18-22°C with diffuse light, until fruiting bodies swell and harvest-ready asci appear.1

Outputs

  • Human food: fruiting bodies tested safe for food use, rich in protein.1
  • Medicinal use: fruiting bodies contain cordycepin, polysaccharides, polyphenols, glycoproteins, superoxide dismutase, and ergosterol.1

Type

Footnotes

  1. Upcycling Chitin Waste and Aged Rice into Fungi Protein Through Fermentation with Cordyceps militaris | Journal of Fungi, 2025 ↩ ↩2 ↩3 ↩4 ↩5 ↩6 ↩7 ↩8