Flammulina velutipes - Enoki

Enoki (Flammulina velutipes) is a cold-tolerant fungus cultivated on hardwood sawdust; grown under light it forms short-stemmed, normally-capped mushrooms, while darkness and cold produce the long, pale stems sold commercially.¹

Inputs

• Substrate: spruce sawdust and rice bran, 3:1 by volume, moistened to 65% water content, packed into culture bottles around a central air hole, and autoclaved (121°C, 20 minutes).¹
• Spawn: sawdust-based spawn colonizes and fruits faster and more heavily than agar-disc spawn.¹
• Spawn run: 25°C in the dark until mycelium fully covers the substrate, about 10-12 days.¹
• Fruiting trigger: after spawn run, scraping off the old surface mycelium (“kinkaki”) and soaking the surface with water promotes more numerous, more evenly-timed fruiting.¹
• Fruiting: 5-10°C; about 30-45 days from inoculation to harvest. Light during fruiting produces short-stemmed, normally-capped mushrooms; constant darkness produces the long, thin-stemmed, pale form.¹

Outputs

• Human food¹

Type

• Edible fungi

Footnotes

1. Togashi, I. and Fukuchi, S. (2022). Enokitake Mushroom (Flammulina velutipes) Cultivation using a Household Electric Refrigerator, Journal of the National Institute of Technology, Asahikawa College, 59, 28-36. ↩ ↩² ↩³ ↩⁴ ↩⁵ ↩⁶ ↩⁷